Synthesis and Characterization of the Superparamagnetic

• Main Authors: Chen, Ming-Hung, 陳明宏
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/17990111694719502983

碩士 === 國立交通大學 === 生物科技學系 === 98 === Molecular imaging has recently been developed very rapidly and extensively in biotechnology. Tumor-targeted drug delivery can enhance the effectiveness of therapy while decreasing the systemic toxicity of these drugs. A new magnetic resonance imaging (MRI) contrast agent containing Erbitux was synthesized and reported. Recent investigations show that manganese doped superparamagnetic iron oxide (MnFe2O4) nanoparticles have higher T2 relaxivity and magnetization than those of iron oxide (Fe3O4) nanoparticles. Therefore, the manganese ferrite nanoparticles (MnFe2O4) were synthesized by thermal decomposition to get higher T2 relaxivity and enhance the negative enhancement. The MnFe2O4 nanoparticles were synthesized and coated with polyethylene glycol (PEG), which has been frequently used as a drug carrier because of its biocompatibility, water solubility, low cytotoxicity, and their ability to escape capture by macrophages. The results of in vitro MR imaging indicated that the negative enhancement of the different cancer cell lines (A431, SKBR-3, PC-3, and COLO-205) are correlated to the EGF receptor expression level. The MnFe2O4-Erbitux nanoparticles were administered to high EGF receptor expression tumor allograft mice by intravenous injection, and the positive tumor was patently detected in T2-weighted MR images as a 23 % enhancement drop indicating a high level of accumulation of the contrast media within the positive tumor. Therefore, targeting of MnFe2O4-Erbitux nanoparticles into positive cells was observed by in vitro and in vivo MR imaging studies.