| Summary: | 碩士 === 國立交通大學 === 生物科技學系 === 98 === Over the past few decades, increasing attention has been paid to synthesize contrast agent for magnetic resonance imaging (MRI). MRI is a non-invasive and high resolution technique that has become a powerful diagnostic tool in medicine. In this study, we designed and synthesized a tryptophan based contrast agent (4S)-4-indolyl-3,6,10-tri(carboxymethyl) -3,6,10-triazadodecanedioic acid (Try-TTDA) and its Gd(III) chelate ([Gd(Try-TTDA)]2?{) that can recognize Cu2+ ion in the living cells. Moreover, the Gd3+ complex attributes excellent selectivity for Cu2+ over a choice of other metal ions. The interaction between Cu2+ and side chain indole of [Gd(Try-TTDA)]2?{ was quenching of intrinsic tryptophan fluorescence. A gradual increases in the relaxivity and signal enhancement of ex-vitro and in vitro MR imaging upon Cu2+ detection. These results implicate that a new MR based contrast agent can serve as a Cu2+ sensor using fluorescence spectrophotometer and MR imaging.
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