| Summary: | 碩士 === 國立交通大學 === 機械工程學系 === 98 === Atmospheric plasma become popular in recent years. It has been used in surface modification, Biomedical Technology, Energy Science, and even environmental engineering. Although not so clean, but the advantage is that low-cost, and fast processing time. therefore, atmospheric plasma technology can be applied very widely. The experiment was made using different proportions of ammonia(5%) /nitrogen mixture to generate plasma, and modify the surface of PLA. We expect to promote the biocompatibility by plasma generated nitrogen-based functional groups such as primary amino groups. The discharge was generated between two planar metal electrodes, with the both electrodes covered by quartz and the PLA sample was placed post-discharge region. At the beginning, plasma properties such as electrical, spectrum, temperature and chemical composition will be measured. To optimize the parameters as the experimental conditions. After plasma treatment, the contact angle changes from 82o to a minimum of 28o. N1s atomic ratio increase from 0% to 2.97%, it’s can be reach 5% with the increasing treatment times. The effect of plasma treatment on cell affinity of PLA surface was identified by observing the morphology through the culture of mouse C2C12 cells. Obviously, many cells attached to the surface of the modified PLA film and the cells stretched very well. Cells activity was grow stable in a long time observation. Compare with FTIR and XPS data, we might be known that primary amino functional groups(399.2 eV) are beneficial to cell adhesion. And how to get the optimize results by experimental control. Aging effect of plasma polymer are important, as marked changed to the surface chemistry of polymer may happen over the storage period. The detail of phenomenon will be described in this paper.
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