| Summary: | 碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 98 === In order to understand the complexity of regulation, expression and feedback mechanism of
genes, the methodology of system biology through the high-throughput analysis and information
analysis must be utilized to elucidate the gene regulation network. The advance in microarray
experiment and its high throughput analysis has set up an important milestone in the system
biology.
In this study, we used the exon array and high throughput data analysis software developed
by Partek Corporation to observe the change of EGFR downstream targets after EGF and
Cetuximab blockade in Sw480, Caco2 and Ht29 cells. By using SAM biochip significance
analysis method, 3052 significantly different genes were selected. These genes were grouped by
different functions and relativity between different cell lines by correlating these genes with
KEGG mapping .We analyze the heat map of each cell line before and after stimulation by using
R package. We also compare the difference of control and experiment group and similarity
between other two groups by using Euclidean distance and Pearson correlation.
Using KegArray in conjunction with KEGG biological information pathway data，we found
the different downstream pathway transformation.of the Sw480、 Ht29 、Caco2 cell line after
treatment with Cetuximab to block EGFR and EGFR-Ras pathway.
In the furture, we hope to detect different gene mutation after treated with Cetuximab using
colon cancer cell lines and observe gene alteration in various EGFR-Ras pathway. We want to
integrate complex gene interaction by using system biology. It is valuable to study and
investigate the regulation mechanism of upstream and downstream interaction in a pathway.
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