Analysis of NGF induced activity of pathways through microarray conjunction with chemical genomics

• Main Authors: CHI CHIEN, 蔡濟謙
• Other Authors: Qing-Dong Ling
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/35379130040109708071

碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 98 === KEGG database is based on networks derived from the interrelation between the molecular biology and the underlying chemical elements. This network database allows scientist to analyze the biological network structure, the genomic information and higher order functional information from a systemic level. We used KEGG database to study the NGF induced neuronal differentiation of PC12 cells through systems biology analysis and annotation of biological function of signal pathway after microarray experiments. In our study, we found that both the overall number of genes or genes associated with neural cell differentiation in the pathway are increased after the NGF stimulation. Activation of Trk signaling pathways of p38 gene gradually increased between 1 and 3 hours , along with the activation of the MAPK signaling pathway. This study, though the experimental data of gene chips and bio-network, tried to explore the feasibility of using biological pathway studies though the high through-put method to study the complex biological systems.