| Summary: | 碩士 === 國立嘉義大學 === 生化科技學系研究所 === 98 === Uropathogenic Escherichia coli (UPEC) strains are the most frequent cause of urinary tract infections (UTI) in humans. Bacterial fimbriae are important virulence factors that allow binding of the bacteria to specific receptors on epithelial cells of the urinary tract. The means by which urinary epithelial cells sense a bacterial infection and which intracellular signaling pathways are activated upon infection are poorly understood. Human bladder epithelial cells will be used to investigate the response to infection with bacteria. We will analyze the Cyclooxygenase-2 (COX-2) expression induced by bacterial infection in human bladder epithelial cells, and investigate the signal transduction pathways involved in COX-2 gene expression. The results indicate that type 1 fimbriated J96 specifically invaded bladder 5637 epithelial cells and induced COX-2 expression. Type 1 fimbriated J96-induced COX-2 induction involved the p38 mitogen-activated protein kinase （MAPK）, as well as Extracellular signal-regulated kinase (ERK), c-Jun N-terminal Kinase (JNK) pathways, which leads to Activating protein-1 (AP-1) and Nuclear factor-kB (NF-kB)-mediated gene expression.
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