Isolation and characterization of oil-rich algae from fish pond

• Main Author: 陳筌鴻
• Other Authors: Wen-Hsing Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/60559914445216423670

碩士 === 國立嘉義大學 === 生物農業科技學系碩士班 === 98 === Since the industrial revolution , long-term use of fossil oil as a fuel source rapidly depletes oil production and supply can not be sustained for long-term needs. Long-term use of fossil fuels also causes environmental pollution and because of CO2 and other greenhouse gases, which caused the Earth's temperature to rise. In addition,the rise of China and India demands significant increases in energy consumption. Therefore, without a significant impact on the current way of life and environmental protection, alternative sources of energy (Alternative energy) must be adopted .Bioenergy would be a good solution. Bioenergy comes from the convertion of organic material into energy, which has the advantages of different forms of environmental protection and sustainability (Renewable). From an efficiency point of view, biodiesel is advanfageous over bioethanol. Therefore, biodiesel is considered a good energy source. Use of microalgae for biodiesel production has the advantage that algae can grow rapidly with low water consumption in comparison to culture of food crops. For all these reasons, it is clear that use of microalgae for biodiesel production in the future can not only absorb carbon dioxide, but also reduce CO2 emission . Algae have a fast rate of growth in comparison to other energy crops. In this study, two algae species, A and B, were isolated from fish pond water and molecular biological analysis revealed that A was identified as a Chlorella sp., but B was not identifiable by this approach. The oil content of algae A (Chlorella sp.) can be as high as 55% while the oil content of algae B can be as high as 70% . Furthermore, by using Agrobacterium for genetic transformation in algae A for expression of foreign proteins, it can increase the commercial value of the microalgae.