Anti-cancer and anti-inflammatory effect of propyl gallate

• Main Authors: Wan-Chen Lin, 林婉蓁
• Other Authors: Ching-Hsein Chen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/79971165548358681799

碩士 === 國立嘉義大學 === 生物醫藥科學研究所 === 98 === Propyl gallate (PG), an ester also known as propyl 3,4,5-trihydroxybenzoate that belongs to the polyphenolic compound family is synthesized by the condensation of propanol and gallic acid. As synthetic antioxidant, PG has been used in processed food and medicinal preparations. The biological activity of propyl gallate is consistent with its free-radical ability, antimicrobial activity, antifungal activity, antibacterial activity, anticariogenesis and anti-inflammatory. Up to now, the anti-cancer effect and anti-inflammatory mechanism of PG are unclear. In the present study, we investigated the effects of PG on the expression of COX-2 and prostgrandin E2 production in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced THP-1 cells. Moreover, PG reduced the levels of p65, a functionally active subunit of NF-B in nuclear fractions prepared from TPA-induced THP-1 cells. PG also inhibited phosphorylation of p65-(Ser-536) and IKK induced by TPA. On the other hand, PG reduced the viability of THP-1, Jurkat, and HL-60 leukemia cells. In addition, we observed DNA condensation, morphology changed and induced apoptosis in THP-1 cells. As a potential chemopreventative agent and anti-inflammatory agent, PG might serve as a food supplement for human leukemia patients.