Molecular cloning and characterization of a nuclear receptor expressed in the reproduction of scleractina coral, Euphyllia ancora

• Main Authors: Ya-Peng Wang, 王雅鵬
• Other Authors: Wen-Hung Twan
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/65908976049800085522

碩士 === 國立東華大學 === 海洋生物科技研究所 === 98 === The objectives of this study were to investigate the steroid hormone receptor which is responsible for reproduction, through molecular cloning of receptor sequences in scleractinian coral, Euphyllia ancora. Full lengths of cDNA encoding a novel nuclear receptor (eaNR) were cloned in the E. ancora by PCR. The 1596 bp eaNR contains 1074 bp open reading frames corresponding to 358 amino residues. The multiple alignment and phylogenetic analyses indicate that identified cnidarian NRs are members of NR subfamily 2. To evaluate the eaNR mRNA expression profiles during coral spawning season, quantitative real-time PCR were taken place to determine the eaNR mRNA amounts. Expression of eaNR mRNA in E. ancora was significantly higher in spawning (May, 2009) and early gonad development. These findings indicate that the eaNR is a nuclear receptor and correlated to the gonad development and timing of mass spawning.