The Effect of Gender on Cardiac Inflammation and Fibrosis after Myocardial Infarction in Mice

• Main Author: 吳家賢
• Other Authors: Hui-Liang Wang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/81627536705139346253

碩士 === 高雄師範大學 === 生物科技系 === 98 === Coronary artery supplies blood to the left vertical, left septum and several chef regions in the left heart. When myocardial ischemic occurs, ischemic heart disease is developed. The aim of the current study is to discover the relationship between the survival rates and gender when acute myocardial infraction attacks. Group 1 and 2 are normal female and male mice; the following two groups are female and male mice receiving ligation of the coronary artery and induced acute myocardial infraction. Group 5 is male mice receiving orchiectomy and induced acute myocardial infraction. The last group is female mice with oophorectomy and induced acute myocardial infraction. There are six mice in each group. The results of the study demonstrated that the group of male mice with ligation of the coronary artery and induced acute myocardial infraction performed the lowest survival rates (30%). In contrast, the female mice with oophorectomy displayed the highest survival rates (90%).Furthermore, the data in HE stain showed that the damage degree caused by infraction was the most serious in male mice with ligation and oophorectomy. According to the study, the data showed a significant difference (P< 0.05) between the male and female mice with induced acute myocardial infraction. It may indicate that female mice might have less detrimental damage than male mice after an acute myocardial infraction attack. In addition, the results from Masson’s trichrome stain demonstrated that the degree of cardiac fibrosis in female mice in experimental groups was more alleviative than in male mice in experimental groups. Therefore, the female mice had lower risk of heart failure and had greater survival rates. Besides, the data of macrophage test displayed that the number of CD40 and CD68 were found greater in male mice in experimental groups. Consequently, worse cardiac inflammation could appear to male mice because of higher macrophage numbers. In MMP-9 test, the gene expression of the four experimental groups was higher than the two control groups; the male mice in experimental groups had higher gene expression than female mice. However, eNOS in the female experimental groups was higher than the male mice. As a result, it could restrain the following contagious heart failure and lead to a better survival rates in female mice. Another gene, IL-10, associated with anti-inflammation was found higher in female mice of experimental groups. It could decline inflammation when a heart is dysfunction as well as could increase survival rates when the verticals are remodeled. Moreover, the female mice in experimental groups might have better capacity to repair damaged heart tissue because TGF-β was higher in the female mice. To sum up, the female mice appeared to have better repair and anti-inflammation abilities. These abilities lead to an increasing survival rates when the vertical are fibrostic. According to the study, it claims that sex hormone plays a crucial role. When the gonads were removed, gonadtropin carried an impact on the progression and survival rates of an acute myocardial infraction attack.