Modular Modern Microfluidic Chip System

• Main Authors: Ming-Hsi Hsieh, 謝明熹
• Other Authors: Chin-Lung Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/60371086557822216981

碩士 === 國立屏東科技大學 === 車輛工程系所 === 98 === 　　In this study, a so-called “Modern Microfluidic Chip System”(MMCS) which is under concept of micro total analysis system (μ-TAS) was designed. MMCS contains three subsystems: “pneumatic propel fluid system”, “chip platform” and “microfluidic detection chip”. Traditional syringe pumps were replaced by the pneumatic propel fluid system propel fluid by compressed air. In order to improve the defects of the traditional syringe pumps, the solenoid valves and pressure regulating valves were used to control the opening-closing and the pressure. The basis of the chip platform is aluminum alloy, and the top of chip platform is PMMA. Chip platform could adjust the Nanoport shortcomings, which is without experimental preparation time and lower cost. The substrate of “microfluidic detection chip” is PMMA, micromachining the microchannel by CO2 laser, with the process faster, easier modification, and without using of photolithography process. The first generation of microfluidic glucose concentration detection chip used the “Double Parallel Connection Micromixer”(DPCM) and T-type fluid outlet micro-channel to mix the solution. The second generation of microfluidic glucose concentration detection chip with addition to the third mixing chamber and serpentine microchannel could increase mixing efficiency. In this study, glucose concentration was detected by DNS reagent method. The DNS reagent and the glucose solution inject into the detected chip and the volume ratio of 1:1, and then the chip was cooling to room temperature after heated, and placed into the spectrophotometer for detected absorbance at the wavelength of 540nm and drew the calibration curve and R2 value of the glucose solution. Hence, MMCS is convenient, simple, quick, no cross-contamination and can be disposable. Using modern microfluidic chip system for detection experiment of glucose concentration, R2 values can reach more than 0.995, the results showed that the “Modern Microfluidic Chip System” can be applied to the actual detection.