| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Diabetes mellitus（DM）is one of the metabolic disorders due to deficiency of insulin action. However, available medicines for diabetes mellitus have a number of limitations, such as adverse effects and high rates of medical failure. Several studies have demonstrated that traditional Chinese medicinal plants contain anti-hyperglycemic activity. The purpose of current study is to evaluate the anti-hyperglycemic activity of Shouzhangshen （Gymnadenia conopsea R. Br.） polysaccharide. The objectives will focus on the capacity of blood glucose regulation by aqueous extract from Gymnadenia conopsea and investigate the safety of Gymnadenia conopsea polysaccharide. The Orchidaceae plant, Gymnadenia conopsea R. Br. is a perennial herb widely distributed in China. The tubers of this plant have been used as a traditional Chinese medicine for the treatment of asthma, neurasthema, and chronic hepatitis. The rats in treatment groups were fed on diets in the form of 5、25、50 mg/Kg B.W. supplement of Gymnadenia conopsea in weight percentile for 10 weeks. The results shows that diabetic rats which received Gymnadenia conopsea had significantly control fasting plasma glucose from 325 mg/dL to 211 mg/dL, especially in the group of a 25 mg/Kg B.W. polysaccharides supplement. After 25 mg/Kg B.W. polysaccharides treatment the area under curve (AUC) decreased 15.96%. Normoglycemic study further revealed the potential of Gymnadenia conopsea to decrease the blood glucose level in normal rats, but do not cause hypoglycemia. Evaluate its safety by Ames test three different dosage of Gymnadenia conopsea polysaccharides, 5, 25 and 50 mg/plate, depicted no toxicity and mutagenicity under specified conditions.
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