Studies on the Natural Products from the Taiwanese Soft Corals Cespitularia taeniata 、Gorgonian Junceella juncea and Taxus sumatrana

• Main Authors: Shih-sheng Wang, 王士昇
• Other Authors: Meng-Hsien Chen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/08192200953151533952

博士 === 國立中山大學 === 海洋生物科技暨資源學系研究所 === 98 === This dissertation mainly discussed the investigation of two different Formosan soft corals, Junceella juncea and Cespitularia taeniata, as well as twigs and needles of Taxus sumatrana (Taxaceae). Their EtOAc extracts were investigated by intensive chromatography. Twenty-seven new natural products were isolated and parts of their biological activities were studied. Three new briarane-type natural products, Juncenolides I-K（1-3）, were isolated from Junceella juncea. In the research of soft coral Cespitularia taeniata, eleven new compounds inculding two new skeleton natural products （4-5）of 6/13 bicyclic, one cespitularane-type diterpene (6), four verticillane-type diterpenes (7-10), one 10, 11-seco verticillane-type diterpene (11), three sesquiterpenes (12-14) were purified. Chemical investigation of Taxus sumatrana (Taxaceae) resulted in isolation of thirteen new taxane diterpenes, including tasumatrols R-U, W, Z （15-18, 20, 23）, six wallifoliol-type diterpenes, tasumatrols V, Y（19, 22） and 11(15→1), 11(10→9) diabeotaxane diterpenes , taiwantaxins B-C （25-26） ; 2(3→20) abeotaxane diterpene, tasumatrol X ( 21）; one 11(15→1) abeotaxane diterpene, taiwantaxin A（24）; one 6/8/6 ring system diterpene, taiwantaxin D（27）,. All the structures of above compounds were elucidated by physical and spectroscopic analyses (IR, mass, UV, optical rotation and NMR), and also by comparisions with the published data. Cytotoxicity and in vitro anti-inflammatory activities were measured by Dr. Kuo Yao-Haur, respectively. The biological activities of compounds 1-14 against MCF-7 (Human breast adenocarcinoma), Doay (Human medulloblastoma), WiDr (Human colon adenocarcinoma), Hela (Human cervical epitheloid carcinoma) , compounds 12 and 14 showd weak cytotoxicity. Tasumatrol Y (22) exhibited mild cytotoxicity against human liver carcinoma (Hepa2) with ED50 at 3.5 μg/mL. Taiwantaxin B (25) showed marked cytotoxicity against human prostate cancer (PC-3) cell lines with an IC50 of 5.02 +0.6 μg/mL (8.96 +0.34 μM).