Effect of frozen storage on the muscle of small abalones (Haliotis diversicolor supertexta)

• Main Authors: Wei-Yo Chen, 陳韋佑
• Other Authors: Ching-Yu Tsao
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/32399861313040479538

碩士 === 國立臺灣海洋大學 === 食品科學系 === 98 === The aim of this study was to investigate the quality of small abalones (Haliotis diversicolor supertexta) during frozen storage. Alive small abalones were washed and freezing with different temperature conditions (-20oC, -40oC), and then frozen storage at -20oC. The samples were taken out for the analyses about the extractable actomyosin, Ca+2-ATPase activity, freshness, water holding capacity and the observations of histological section. From the results, the percentage of extractable actomyosin decreased to 3.69% at -20oC group in the sixth month and about maintained 4.3% at -40oC group in the same time; Ca+2-ATPase specific activity decreased to 0.17 and 0.24 μmole Pi/min/mg protein, respectively. In water holding capacity, the former decreased to 21% and the latter kept stable. And the volatile basic nitrogen of the former had the highest value (6.46%) which was still belongs to freshness. The total –SH group of two groups were found no significant difference with extending storage, but the reactive –SH group decreased. And, the reactive –SH group decreasing trend in -40oC freezing condition is slighter than -20oC. This appearance was due to the protein molecules became larger via aggregation so that the –SH group were buried. From observations of histology, it was found that the muscle fiber in two groups had twisted, and the gaps between muscle fibers had increased with extending frozen storage time.