Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells
碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 98 === Epithelial cell adhesion molecule (EpCAM) is a cell adhesion molecule and a potential target for therapeutic antibodies. Recently, EpCAM has been shown to regulate tumor cell proliferation by its nucleocytoplasmic intracellular domain fragment (ICD). EpCAM intr...
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ndltd-TW-098NTOU56130172015-10-13T19:35:33Z http://ndltd.ncl.edu.tw/handle/47656085868312770304 Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells 上皮細胞附著因子(EpCAM)與緊密結合蛋白因子(tight junction protein claudin-7)於反程序化老鼠成體細胞為誘導型全分化潛能幹細胞時所扮演的角色 Yao-De Huang 黃耀德 碩士 國立臺灣海洋大學 生物科技研究所 98 Epithelial cell adhesion molecule (EpCAM) is a cell adhesion molecule and a potential target for therapeutic antibodies. Recently, EpCAM has been shown to regulate tumor cell proliferation by its nucleocytoplasmic intracellular domain fragment (ICD). EpCAM intracellular domain (EpICD) activates C terminal myelocytomatosis oncogene (c-Myc) in nucleus by Wnt signal pathway. On the other hand, the complex of EpCAM and the tight junction protein claudin-7 (CLDN-7) would promote tumorigenicity and accelerates tumor growth. We observed that EpCAM and CLDN-7 express in both mouse embryonic stem cells and induced poluripotent stem cells. In this study we first discovered that the expressions of EpCAM and CLDN-7 would be activated when reprogramming mouse embryonic fibroblast cells into induced pluripotent stem cells. The efficiency of iPS reprogramming was enhanced by overexpressing EpCAM, CLDN-7 or both. Furthermore, silence of endogenous EpCAM or CLDN-7 resulted in the reduction of iPS reprogramming. However, ectopic expression of EpCAM and CLDN-7 in differential somatic cells such as mouse fibroblast cells had no effect on the activation of pluripotency-associated gene Nanog, Oct4, Sox2, c-Myc and KLF4. Our data show that EpCAM positively regulates iPS reprogramming, yet the detail mechanism remains unclear. Hung-Chih Kuo 郭紘志 2010 學位論文 ; thesis 85 zh-TW |
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碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 98 === Epithelial cell adhesion molecule (EpCAM) is a cell adhesion molecule and a potential target for therapeutic antibodies. Recently, EpCAM has been shown to regulate tumor cell proliferation by its nucleocytoplasmic intracellular domain fragment (ICD). EpCAM intracellular domain (EpICD) activates C terminal myelocytomatosis oncogene (c-Myc) in nucleus by Wnt signal pathway. On the other hand, the complex of EpCAM and the tight junction protein claudin-7 (CLDN-7) would promote tumorigenicity and accelerates tumor growth. We observed that EpCAM and CLDN-7 express in both mouse embryonic stem cells and induced poluripotent stem cells. In this study we first discovered that the expressions of EpCAM and CLDN-7 would be activated when reprogramming mouse embryonic fibroblast cells into induced pluripotent stem cells. The efficiency of iPS reprogramming was enhanced by overexpressing EpCAM, CLDN-7 or both. Furthermore, silence of endogenous EpCAM or CLDN-7 resulted in the reduction of iPS reprogramming. However, ectopic expression of EpCAM and CLDN-7 in differential somatic cells such as mouse fibroblast cells had no effect on the activation of pluripotency-associated gene Nanog, Oct4, Sox2, c-Myc and KLF4. Our data show that EpCAM positively regulates iPS reprogramming, yet the detail mechanism remains unclear.
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Hung-Chih Kuo |
author_facet |
Hung-Chih Kuo Yao-De Huang 黃耀德 |
author |
Yao-De Huang 黃耀德 |
spellingShingle |
Yao-De Huang 黃耀德 Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
author_sort |
Yao-De Huang |
title |
Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
title_short |
Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
title_full |
Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
title_fullStr |
Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
title_full_unstemmed |
Roles of EpCAM and CLDN-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
title_sort |
roles of epcam and cldn-7 in reprogramming mouse fibroblast into induced pluripotent stem cells |
publishDate |
2010 |
url |
http://ndltd.ncl.edu.tw/handle/47656085868312770304 |
work_keys_str_mv |
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