Target Fluorescent Probes for Detecting Hydrogen Peroxide in Macrophage Cell

• Main Authors: Ching-Min Chang, 張靖敏
• Other Authors: Shih-Hsiung Wu
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/77722282994735171162

碩士 === 國立臺灣大學 === 生化科學研究所 === 98 === One major contributor to oxidative damage is hydrogen peroxide (H2O2), which has been considered mostly as reactive oxidative species (ROS), and mediate pathogenic processes. In addition, H2O2 is emerging as a newly recognized as a secondary messenger in cellular signal transduction. However, a substantial challenge in elucidating its diverse roles in complex biological environments is the lack of methods for probing this reactive oxygen metabolite in living systems specifically. Here, we reported the synthesis and application of Probe 1 (1a and 1b), two new fluorescent probes that showed high selectivity for H2O2 and were capable of visualizing the addition of exogenous H2O2 to living cells. These probes were able to serve as a tool for evaluating the H2O2 production in complex physiological and pathological processes. The main purpose of this study was to create an easy, time-saving, and a straightforward platform for measurement the exact H2O2 concentration in living RAW 264.7 cell. Through quantitating the relative fluorescence intensity in vivo by spectrofluoromerty, we might discover new small molecules for potential antioxidants in the future.