| Summary: | 碩士 === 臺灣大學 === 昆蟲學研究所 === 98 === Imidacloprid is one of systemic insecticides. The residue of imidacloprid in the nectar and pollens of the plants would be poisonous not only to the honeybee adults but also larvae. It has been shown that the development of the honeybee larvae were impeded if the larvae were fed with imidacloprid contaminated nectar or pollen collected by bee workers. However, our understanding on the risk of imidacloprid to honeybee larvae is still in a primitive stage. In this study, the survival, capped-brood, pupation and eclosion rates were recorded after treating the larvae directly in the hive with imidacloprid in different dosages. The larvae brood-capped rates significantly decreased when the dosages increased from 2000 to 8000 ng, but no significant effect on the survival, brood-capped, pupation and eclosion rates when treated with DMSO or 0.4-200 ng imidacloprid. Although the sub-lethal dosage of imidacloprid had no effect on the eclosion rate, the learning ability of the poisoned bees tested with the classical olfactory conditioning, proboscis extension reflex. The result showed the larvae treated with 0.04 ng imidacloprid, the learning ability of the poisoned bees were impaired. The result suggests that the sub-lethal dosage of imidacloprid damage the development of nervous system and the foraging ability.
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