Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting

• Main Authors: Ting-Yun Cheng, 鄭婷云
• Other Authors: 楊台鴻
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/56331337554649706222

碩士 === 國立臺灣大學 === 高分子科學與工程學研究所 === 98 === Generally, particle uptake by cells is mediated by electrostatic interactions of positively charged particles with negatively charged cellular surface, so the cationic polyplexes showed efficiently cellular transfection. However, YC Chung et al. developed a new complex with high transfection efficiency by assembling negatively charged single-stranded oligonucleotides with polycation/DNA complexes. Therefore, this project investigated the cellular uptake pathway of single-stranded oligonucleotides with polycation/DNA complexes. The results of different inhibitors showed these complexes can cellular uptake by several pathways. However, only the caveolae and macropinocytosis pathway would influence gene expression. Furthermore, iron oxide particles will be investigated by single-stranded oligonucleotide-assembled technique. The results indicate the oligonucleotide-coated iron oxide particles could be uptaken and present low cytotoxicity. This technique of oligonucleotide-assembled iron oxide particles can easily manufacture and without further purification. To integrate the oligonucleotide-coated iron oxide particles and cellular uptake pathway, the results also shows the oligonucleotide-coated iron oxide particles could be internalized by several pathways. Finally, this project successfully develop a multifunctional iron oxide platform by conjugating oligonucleotides, and also develop the ligand or fluorescent probe by nucleic acid base-paired interaction. Therefore, the multifunctional iron oxide particles can be applied to MRI or fluorescent target to track tumor in vivo.