Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting
碩士 === 國立臺灣大學 === 高分子科學與工程學研究所 === 98 === Generally, particle uptake by cells is mediated by electrostatic interactions of positively charged particles with negatively charged cellular surface, so the cationic polyplexes showed efficiently cellular transfection. However, YC Chung et al. develope...
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ndltd-TW-098NTU053101162015-11-02T04:04:00Z http://ndltd.ncl.edu.tw/handle/56331337554649706222 Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting 探討單股核酸包覆奈米粒子於吞噬機制及細胞標定之應用 Ting-Yun Cheng 鄭婷云 碩士 國立臺灣大學 高分子科學與工程學研究所 98 Generally, particle uptake by cells is mediated by electrostatic interactions of positively charged particles with negatively charged cellular surface, so the cationic polyplexes showed efficiently cellular transfection. However, YC Chung et al. developed a new complex with high transfection efficiency by assembling negatively charged single-stranded oligonucleotides with polycation/DNA complexes. Therefore, this project investigated the cellular uptake pathway of single-stranded oligonucleotides with polycation/DNA complexes. The results of different inhibitors showed these complexes can cellular uptake by several pathways. However, only the caveolae and macropinocytosis pathway would influence gene expression. Furthermore, iron oxide particles will be investigated by single-stranded oligonucleotide-assembled technique. The results indicate the oligonucleotide-coated iron oxide particles could be uptaken and present low cytotoxicity. This technique of oligonucleotide-assembled iron oxide particles can easily manufacture and without further purification. To integrate the oligonucleotide-coated iron oxide particles and cellular uptake pathway, the results also shows the oligonucleotide-coated iron oxide particles could be internalized by several pathways. Finally, this project successfully develop a multifunctional iron oxide platform by conjugating oligonucleotides, and also develop the ligand or fluorescent probe by nucleic acid base-paired interaction. Therefore, the multifunctional iron oxide particles can be applied to MRI or fluorescent target to track tumor in vivo. 楊台鴻 2010 學位論文 ; thesis 63 zh-TW |
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碩士 === 國立臺灣大學 === 高分子科學與工程學研究所 === 98 === Generally, particle uptake by cells is mediated by electrostatic interactions of positively charged particles with negatively charged cellular surface, so the cationic polyplexes showed efficiently cellular transfection. However, YC Chung et al. developed a new complex with high transfection efficiency by assembling negatively charged single-stranded oligonucleotides with polycation/DNA complexes. Therefore, this project investigated the cellular uptake pathway of single-stranded oligonucleotides with polycation/DNA complexes. The results of different inhibitors showed these complexes can cellular uptake by several pathways. However, only the caveolae and macropinocytosis pathway would influence gene expression.
Furthermore, iron oxide particles will be investigated by single-stranded oligonucleotide-assembled technique. The results indicate the oligonucleotide-coated iron oxide particles could be uptaken and present low cytotoxicity. This technique of oligonucleotide-assembled iron oxide particles can easily manufacture and without further purification. To integrate the oligonucleotide-coated iron oxide particles and cellular uptake pathway, the results also shows the oligonucleotide-coated iron oxide particles could be internalized by several pathways.
Finally, this project successfully develop a multifunctional iron oxide platform by conjugating oligonucleotides, and also develop the ligand or fluorescent probe by nucleic acid base-paired interaction. Therefore, the multifunctional iron oxide particles can be applied to MRI or fluorescent target to track tumor in vivo.
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楊台鴻 |
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楊台鴻 Ting-Yun Cheng 鄭婷云 |
author |
Ting-Yun Cheng 鄭婷云 |
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Ting-Yun Cheng 鄭婷云 Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
author_sort |
Ting-Yun Cheng |
title |
Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
title_short |
Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
title_full |
Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
title_fullStr |
Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
title_full_unstemmed |
Investigating the Oligonucleotide-assembled Nanocomplexes on Intracellular Uptake and Application of Cell Targeting |
title_sort |
investigating the oligonucleotide-assembled nanocomplexes on intracellular uptake and application of cell targeting |
publishDate |
2010 |
url |
http://ndltd.ncl.edu.tw/handle/56331337554649706222 |
work_keys_str_mv |
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