The Study of Somatic Reprogramming of Mouse Pulmonary Stem/Progenitor Cells

• Main Authors: Yu-Chi Wang, 王育婍
• Other Authors: 林泰元
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/70655568822594929379

碩士 === 臺灣大學 === 藥理學研究所 === 98 === Adult stem cells are distributed in many tissues and are responsible for regeneration and repair of specific tissues. Although more than 11 types of lung adult stem cells have been identified, few of them can be efficiently isolated and expanded in vitro. We have previously developed a serum-free mouse Oct4＋ pulmonary stem/progenitor cell (mPSC) culture system. These cells were multipotent and susceptive to SARS coronavirus, influenza A virus and Herpes Simplex virus type 1, suggesting their potential in basic research and clinic application. However, mPSCs could not be expanded in a large scale. Here, we used somatic reprogramming (i.e. transducing Oct4, Sox2, Klf4, and c-Myc into somatic cells) to generate an immortal mPSC (imPSC) line and maintain their multipotency. Our data demonstrate that the phenotype of imPSCs was comparable to which of primary mPSCs. Furthermore, imPSCs showed high telomerase activity and the capability of pulmonary differentiation. Importantly, they displayed vigorous proliferation and teratoma formation, suggesting that the cancerous transition of normal mPSCs could be triggered and facilitated by somatic reprogramming. Our work proposes that imPSCs could be generated by somatic reprogramming and served as a feasible line for the design of experimental and therapeutic approaches in virus infection and respiratory diseases.