Development of a biological tissue slide scanning instrument using a line scan CCD camera

• Main Authors: Ming-sheng Hung, 洪明勝
• Other Authors: Fang-jung Shiou
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/12839234185145287710

碩士 === 國立臺灣科技大學 === 機械工程系 === 98 === A high speed biological tissue slide scanning instrument has been developed to capture continuous images using a line scan CCD camera and to complete a panoramic stitching in real time. The panoramic image provides a highly reliable reference data for pathological researchers to determine the pathogenic causing of biological tissues. The developed PC-based instrument consists of mainly the mechanical design of the scanning device, the motion control device, an image processing system, and the human-machine interface. This study aims to increase the scanning speed and to obtain more actual images of the biological tissues of the developed instrument. The errors resulting from the positioning mechanism, the diverse geometric features of tissues, and environment-induced factors would make defocused images, loss of image information, and generation of a stitching line. Using the image processing techniques, the auto-focusing methods for tissues images have been investigated to overcome the small depth of field problems. Moreover, a pattern matching technique for stitching tissue images to form a panoramic image has been proposed to achieve fast with high-quality scans of biological tissues.