| Summary: | 碩士 === 東吳大學 === 微生物學系 === 98 === Endocrine disrupting chemicals (EDCs) which are xenobiotic chemicals such as phthalate esters (PAEs) can interfere with the endocrine functions of orginasms. In this study, the expressed sequence tag (ESTs) system developed from shrimp Neocaridina denticulate and four PAEs of aquatic pollutants, including diethyl phthalate (DEP), dihexyl phthalate (DHP), dipropyl phthalate (DPrP) and diphenyl phthalate (DPP), were used to study the effect of PAEs on physiological functions of N. denticulate and to assess the feasibility of the ESTs system to monitor the pollution of aquatic environment. After shrimps were separately immersed four PAEs with different concentrations (0 mg/L, 0.1 mg/L, 1.0 mg/L and 10.0 mg/L of pond water) for one day, the expression of 28 ESTs were detectd by semi-quantitative RT-PCT. This result showed that, comparing with the control group, 13, 16, 2 and 9 ESTs of them were significantly changed in DEP-, DPrP-, DHP- and DPP-treated groups, respectively; among them, the effect of DEP, DPrP and DPP on the functional known ESTs are similar, mainly including both the defence-related and metabolism-related ESTs, MQ protein (FL592852), hemocyanin (FL640908), heat shock 70 kD protein cognate (FL640930), glutathione S-transferase class mu (FL640931) and dehydrogenase 1 (FL640932), mitochondrion (FL640918), elongation factor 1-alpha (FL640913), cathepsin-L like cysteine protease mRNA (FL640915), glutamata dehydrogenase (FL640917), trehalose-6- phosphate synthase 1 (FL640934). As for DHP, its effect on shrimp was lower than othe three PAEs.
To determine the sensitivity of the tested ESTs to the four PAEs, shrimps were immersed in lower dosages (0.01 mg/L and 0.05 mg/L). There were 7 ESTs in 0.01-mg/L-treated groups that the expression significantly changed when was statistically analysed at p<0.05, but 10 ESTs at p<0.1. When the expressed-ratio of ESTs larger or lower than that of control 50 % is decided as the standard, it was suggested that 14 of 28 ESTs could be use as biological molecule indicators.
According to the results, the study indicated that the ESTs system developed from N. denticulate can be used as a tool in studying the effect of environment pollution but also in monitoring the hidden risk of aquatic pollutants.
|
|---|
