| Summary: | 碩士 === 南台科技大學 === 生物科技系 === 98 === Diabetic nephropathy (DN) is one of the major diseases that results in chronic kidney disease (CKD). It is also a common and serious complication of diabetes mellitus, which leads to renal failure in up to 30% of individuals with diabetes and thus becomes the most important end-stage renal diseases (ESRD). In the study, we look for constructing several kinds of non-genetic deficient diabetic nephropathy (DN) mouse models. It is suggested that disease models prior to serving with renal fibrosis, which may be utilized for advanced studies on DN animal models. A nongenetic model of type 2 diabetes has been described that involves feeding rats a high fat diet and administering a low dose of STZ. Therefore, it is important to establish a strain-appropriate STZ dosage protocol for achieving hyperglycaemia before embarking on a major study of diabetic nephropathy using this model. Restricting dietary protein is the one of major components of therapy in CKD patients and aims to slow the progression of renal failure and to provide optimum nutritional status. Many authors have demonstrated that low-protein diet (LPD) and very low-protein diet (VLPD) mixture of EAA ketoanalogs, which are used to prevent nutritional deficiencies caused by protein-restricted diets. It contains insulin resistance, hyperlipidemia and delay the start of renal replacement.
Antrodia camphorata (AC) is a unique fungal species that used as a folk medicine, has attracted extreme attention for inflammation syndromes and liver-related diseases research in Taiwan. It has been known that AC can play the role on anti-oxidation, has recently been marketed in the forms of nutraceuticals. It has been suggested that AC protects the kidney from auto-immune disease. However, the ability of AC to DN has not been subjected to scientific scrutiny.
This study investigated the non-genetic deficient DN animal models, which closely simulates the metabolic abnormalities of the human disease, and it is also been available by cost-effective. The hyperglycemia and hyperinsulinaemia were induced on these HFD-controlled C57BL/6 mice (fasting blood glucose over 180 mg/dl, and ACR over 60 g/mg, alternatively). We also evaluated the metabolic profiles, renal histology and the mechanism of AC treated mice in DN models. We found that the AC has improve the glucose tolerance and increasing blood high density lipoprotein-low density lipoprotein ratio. Immunohistochemical evaluation also showed weak TGF-β and α-SMA staining in the renal glomeruli of native and AC group, while the signal revealed markedly stronger in the control group. In conclusion, our data shows a new non-genetic deficient DN animal models, and prove that the alcoholic extract from AC treatment has shown a potential anti-fibrosis effect in HFD-induced diabetic nephropathy mice.
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