Investigating the role of granulocyte-colony stimulating factor treating on spinal cord injury rats

• Main Authors: CHUNG CHI-RUNG, 鍾啟榮
• Other Authors: 陳勝咸
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/54124014493028439095

碩士 === 南台科技大學 === 生物科技系 === 98 === Our previous results successfully showed that human umbilical cord blood (HUCB) derived hematopoietic stem (CD34+) cells resuscitate and restore heatstroke , spinal cord injury and traumatic brain injury rats. It implicated that CD34+ cells can reduce mortality and injury during acute inflammatory and ischemic injury disease. Many researchers have demonstrated that HUCB cells transplantation can attenuate morbidity of neurodegenerative disease. Recently, a series of studies have demonstrated the neuroprotective effect of Granulocyte-colony stimulating factor (G-CSF) in cerebral ischemia. Moreover, G-CSF exerts neuroprotective effects through different mechanisms, including mobilization of hematopoietic stem cells, anti-apoptosis, neuronal differentiation, angiogenesis and anti-inflammation . Hence, G-CSF not only inhibits neuron death, but also generates "new" neural tissue formation. However, the precise mechanisms of the neuroprotective effect of G-CSF are not entirely known, especially in another ischemia injury diseases. According to these evidences, we can speculate that G-CSF, being used either alone or in combination with another agent, should be an effective strategy in the treatment of ischemia injury victims. However, the roles of G-CSF via different mechanisms causing protective effects during spinal cord injury should be explored. For this reason, we set up a spinal cord injury (SCI) animal model. The aim of this current study was designed to investigate the effects of G-CSF on spinal cord ischemia, cell deaths and injury markers after these insults happened in rats then we administer G-CSF to the rats after these insults immediately. We use B.B.B locomotor score to evaluate hind limb functional recovery in spinal cord injury rats.The terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) assay were conducted at day 7 after insults to evaluate spinal cord ischemia and apoptosis, respectively. We analyze the injury spinal cord tissue tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) via ELISA to detect the levels of endothelial damage and inflammatory exchange. Finally, we successful demonstrated that G-CSF therapy on the experimental spinal cord injury can reduce morbidity of SCI , decrease cell deaths, attenuate inflammation cytokines marker and improve rats hind limb locomotor function.. Keywords：G-CSF, spinal cord injury, endothelial progenitor cell, CD34+ cell, angiogenesis, inflammation, B.B.B locomotor score.