A study of serum free medium for Maruca vitrata cell
碩士 === 南台科技大學 === 生物科技系 === 98 === Requirement of eliminating serum from cell culture have intensified in recent years, with the pressure of regulatory agencies related to biopharmaceuticals production. In this work, A NTU-MV cell line derived from Maruca vitrata, was used to ascertain for the devel...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2010
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| Online Access: | http://ndltd.ncl.edu.tw/handle/78599412302017828617 |
| Summary: | 碩士 === 南台科技大學 === 生物科技系 === 98 === Requirement of eliminating serum from cell culture have intensified in recent years, with the pressure of regulatory agencies related to biopharmaceuticals production. In this work, A NTU-MV cell line derived from Maruca vitrata, was used to ascertain for the development of serum-free medium.
From this ststistical analysis, glucose、glutamine、yeastolate、protein A、lipid、pluronic F-68、lactalbumine were identified as important determinants for cell growth. The adaptation period from convention 5% FBS to SFM was spent about 2000 hrs. The MaviMNPV productivity of MV cell line cultured with serum-free medium was the same with that cultured with conventional serum medium. Subsequently, the composion concentrations of serum-free medium were optimized using the Taguchi methods.
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