Angiogenic Effect of Dammarane Oligo-Sapogenin in Rat Skeletal Muscle after Exhaustive Exercise

• Main Authors: Yang, Chia-Chen, 楊家蓁
• Other Authors: Kuo, Chia-Hua
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/59576927522834692868

碩士 === 台北巿立體育學院 === 運動科學研究所 === 99 === Purpose: The present study was aimed to investigate the effect of Dammarance Oligo-Sapogenin (DS) on angiogenesis and inflammatory markers after exhaustive exercise in rat skeletal muscle. Methods: Sprague Dawley rats (n=100) were equally assigned into 5 groups: placebo, DS, DS20, DS60 and DS120. Respective doses of bioactive compound DS (0.1, 20, 60 and 120 mg/kg body weight) were orally administered to DS groups for 10-week. Ten rats from each group performed a single bout of exhaustive swimming exercise and then muscle tissue was excised immediately after exercise. Results: Plantaris muscle VEGF, PGC-1α and IL-6 mRNA expression were significantly (p<.05) increased after exhaustive exercise in placebo group. phospho-AMPK protein level in DS20 was significantly (p<.05) increased after exhaustive exercise compare to placebo exercised rats. PGC-1α mRNA expression showed decreased trend in DS20, DS60, DS120 groups after exercise compared to placebo exercise group, while PGC-1α protein was significantly (p<.05) decreased only in DS60 and DS120 groups. VEGF protein level was decreased in DS20, DS60 and DS120 groups after exhaustive exercise. We observed no change in Angiopoeitins (Ang-1 and Ang-2) mRNA and protein levels after exhaustive exercise in DS pre-treated rats compare to placebo exercise in both muscle types. However, increased IL-6 mRNA expression after exhaustive exercise was controlled in DS20, DS60 and DS120 pre-treated rats in both muscle types. Conclusions: This study results conclude that Dammarance Oligo-Sapogenin supplementation can attenuate stress response in PGC-1α and VEGF by exhaustive exercise. Furthermore, increased inflammatory marker IL-6 after exhaustive exercise was controlled in the DS pre-treated rats.