Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery

博士 === 國立陽明大學 === 醫學工程研究所 === 98 === Urolithiasis is a common disease with high recurrent rate. Identification of urinary stone composition is important for metabolic evaluation, decision-making and diet control. Traditional urinary stone analytic methods need large stone fragments for analysis....

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Main Authors: Yi-Chun Chiu, 邱逸淳
Other Authors: Huihua Kenny Chiang
Format: Others
Language:en_US
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/86290254162177166314
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spelling ndltd-TW-098YM0055300082015-10-13T18:45:25Z http://ndltd.ncl.edu.tw/handle/86290254162177166314 Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery 拉曼光譜應用於微創手術後尿路結石成分定性與定量的分析 Yi-Chun Chiu 邱逸淳 博士 國立陽明大學 醫學工程研究所 98 Urolithiasis is a common disease with high recurrent rate. Identification of urinary stone composition is important for metabolic evaluation, decision-making and diet control. Traditional urinary stone analytic methods need large stone fragments for analysis. However, with the advancement of ureteroscopic lithotripsy (URSL) or extracorporeal shockwave lithotripsy (ESWL) has resulted in micro stones and unapparently expelled urinary stone powders. We aim to develop a micro-Raman Spectroscopy (MRS)-based method for detecting the composition of micro-stones or stone powders in urine after minimal invasive urological surgery (MIUS). First we establish the Raman spectra database for prevalent standard compounds and then we measure the post operative stones. MRS-based approach reveals comparable and even more detailed analytic result than Fourier transform infrared spectroscopy (FTIR). We also successfully analyze the composition of urinary stone powders directly from the urine samples in post-URSL urine. And then quantitative analysis is based on the construction of calibration curves of known mixtures of synthetically prepared standard compounds. The various concentration ratio of binary mixtures were produced and used as the basis for the quantitative analysis. Intensities of the characteristic bands were used for calculation. The ratios of the relative intensities of Raman bands corresponding to binary mixtures of known composition and content concentration yielded a linear dependence. This bench-to-besides MRS-based approach provides a quick and convenient method for micro urinary stone analysis in both qualitative and quantitative aspect. Huihua Kenny Chiang 江惠華 2010 學位論文 ; thesis 82 en_US
collection NDLTD
language en_US
format Others
sources NDLTD
description 博士 === 國立陽明大學 === 醫學工程研究所 === 98 === Urolithiasis is a common disease with high recurrent rate. Identification of urinary stone composition is important for metabolic evaluation, decision-making and diet control. Traditional urinary stone analytic methods need large stone fragments for analysis. However, with the advancement of ureteroscopic lithotripsy (URSL) or extracorporeal shockwave lithotripsy (ESWL) has resulted in micro stones and unapparently expelled urinary stone powders. We aim to develop a micro-Raman Spectroscopy (MRS)-based method for detecting the composition of micro-stones or stone powders in urine after minimal invasive urological surgery (MIUS). First we establish the Raman spectra database for prevalent standard compounds and then we measure the post operative stones. MRS-based approach reveals comparable and even more detailed analytic result than Fourier transform infrared spectroscopy (FTIR). We also successfully analyze the composition of urinary stone powders directly from the urine samples in post-URSL urine. And then quantitative analysis is based on the construction of calibration curves of known mixtures of synthetically prepared standard compounds. The various concentration ratio of binary mixtures were produced and used as the basis for the quantitative analysis. Intensities of the characteristic bands were used for calculation. The ratios of the relative intensities of Raman bands corresponding to binary mixtures of known composition and content concentration yielded a linear dependence. This bench-to-besides MRS-based approach provides a quick and convenient method for micro urinary stone analysis in both qualitative and quantitative aspect.
author2 Huihua Kenny Chiang
author_facet Huihua Kenny Chiang
Yi-Chun Chiu
邱逸淳
author Yi-Chun Chiu
邱逸淳
spellingShingle Yi-Chun Chiu
邱逸淳
Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
author_sort Yi-Chun Chiu
title Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
title_short Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
title_full Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
title_fullStr Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
title_full_unstemmed Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery
title_sort qualitative and quantitative micro raman spectroscopy identification of urinary stone composition after minimal invasive urological surgery
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/86290254162177166314
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