Qualitative and Quantitative Micro Raman Spectroscopy Identification of Urinary Stone Composition after Minimal Invasive Urological Surgery

• Main Authors: Yi-Chun Chiu, 邱逸淳
• Other Authors: Huihua Kenny Chiang
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/86290254162177166314

博士 === 國立陽明大學 === 醫學工程研究所 === 98 === Urolithiasis is a common disease with high recurrent rate. Identification of urinary stone composition is important for metabolic evaluation, decision-making and diet control. Traditional urinary stone analytic methods need large stone fragments for analysis. However, with the advancement of ureteroscopic lithotripsy (URSL) or extracorporeal shockwave lithotripsy (ESWL) has resulted in micro stones and unapparently expelled urinary stone powders. We aim to develop a micro-Raman Spectroscopy (MRS)-based method for detecting the composition of micro-stones or stone powders in urine after minimal invasive urological surgery (MIUS). First we establish the Raman spectra database for prevalent standard compounds and then we measure the post operative stones. MRS-based approach reveals comparable and even more detailed analytic result than Fourier transform infrared spectroscopy (FTIR). We also successfully analyze the composition of urinary stone powders directly from the urine samples in post-URSL urine. And then quantitative analysis is based on the construction of calibration curves of known mixtures of synthetically prepared standard compounds. The various concentration ratio of binary mixtures were produced and used as the basis for the quantitative analysis. Intensities of the characteristic bands were used for calculation. The ratios of the relative intensities of Raman bands corresponding to binary mixtures of known composition and content concentration yielded a linear dependence. This bench-to-besides MRS-based approach provides a quick and convenient method for micro urinary stone analysis in both qualitative and quantitative aspect.