| Summary: | 碩士 === 國立中正大學 === 化學工程研究所 === 99 === Erythropoiesis is a vital process to the human health. It regulates the appropriated supply of respiratory gases to support the life of human body. Erythropoiesis is a multistep process of the pluripotent hematopoietic stem cells differentiation into mature red blood cells. However, the molecular regulation of erythropoiesis remains to be clarified. In this study, we applied an three-stage erythropoiesis protocol for in vitro culture of human hematopoietic stem cells (HSCs) and a human leukemia cell line, KU812F. The differentiation process of hematopoietic stem cells were monitored using the antibodies for CD71 and glycophorin A (GPA). We found that the growth rate of the purchased HSCs was 15 folds slower than that of fresh purified HSCs. When cocultured with human or rat mesenchymal stem cells (MSC), KU812F cells showed a quicker decrease of CD71 but a similar reduction rate for GPA. Furthermore, rat MSCs was more potent than human MSCs in promoting the fading of CD71. After the differentiation process, the cells were reduced to about 4 μm and no hemoglobin was detected.
|
|---|
