Molecular Identification on Hydrogen Production Microbial Community in Anaerobic Swine Wastewater and Sludge Co-digester

• Main Authors: Lee, Yuhung, 李育宏
• Other Authors: Li,Shieyi
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/77851953903517125306

碩士 === 長榮大學 === 職業安全與衛生學系碩士班 === 99 === The sludge samples are from sewage sludge and swine industry with treatment to compare single-phase to two-stages-reactors in anaerobic co-digestion for discussion of whether acid can helpful to accelerate the improve methane formation and reduce sludge digestion efficiency. There are (1) the swine waste anaerobic sludge acid added and without acid addition treatment (2) acid and acid-free treatments of living sewage sludge and swine manure anaerobic (3) acid added treatment of Two-Stages Process in Anaerobic sludge from living sewage sludge and swine sludge for discussion of the acid added and acid-free treatment of microbial flora of different types of operating factors and a number of increased DNA by the use of PCR were planted cells into the bacterial DNA by genetic screening technology to complete the transformation and detect target bacteria samples for outsourcing sequencing strain and comparison of the similarities and differences between the made micro-organisms based on 16S rRNA gene. Once the inlet sludge TS concentration is controlled at 15,000 mg/l, in the anaerobic co-digestion experiments, DCOD and TVS digestibility are 70%, 40% or more, 109 ml CH4/g TCODremoved, which is higher than DCOD and TVS 40% and 10% of the acid-free One-Stages Process treatment. And it’s 30% and 5% higher than acid One-Stages-Reactors DCOD and TVS. The acid Two-Stages Process experiment has the result 30% higher than One-Stages Process experiment. There are six kinds of sludge dominant bacteria divided, (1) acid added swine acid sludge (2) acid-free swine sludge (3) acid added sewage plant sludge and pig manure (4) without the addition of acid sewage sludge and swine sludge (5) acid added treatment of living sewage and swine Two-Stages Process acetogenesis reactor sludge and (6) acid added treatment of living sewage and swine Two-Stages Process methanogenesis reactor sludge. The revealed complex microbes, because the real field experiments in this study caused the difference in micro aspects. It can also be leaned that the results observed in the anaerobic digestion of swine sludge has benefit bacteria of Arcobacter spp., Rikenella spp. and Sulfurovum spp. It can also be leaned that the results observed in the anaerobic digestion of acetogenesis reactor has benefit bacteria of Castellaniella spp.、Arcobacter spp. and Escherichia/Shigella spp.. It can also be leaned that the results observed in the anaerobic digestion of a methanogenesis reactor has bacteria of Rikenella spp..