| Summary: | 碩士 === 中國醫藥大學 === 基礎醫學研究所碩士班 === 99 === Stem cell therapy and the hope for myocardium and vascular regeneration has become the focus of many basic and clinical investigations for more than a decade. Wharton''s jelly stem cells (WJCs) has self-renew, multipotent differentiation potential and represents an attractive cell source for regeneration of damaged tissues. The implantation of artificial blood vessels and repair damaged tissue has an important capacity. The nanocomposites from polyurethane (PU) containing small amount (43.5 ppm) of gold (Au) nanoparticles was prepared. Previous studies showed that the nanocomposites (PU-Au) exhibited greater proliferation and biological performance of endothelial cells. In this study, we focus on the novel gold nanocomposites whether they can promote the migration and proliferation of WJCs. On the other hand, we collected the endothelial progenitor cells (EPCs) from peripheral blood mononuclear cells (PBMNCs) and cultured on PU-Au can effectively improve the response capacity of vascular tissue damage in vivo. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1, CXCR4 specific ligand) enhanced WJCs migration and proliferation has been observed in this study. Indeed, PU-Au can be modulated signal through the activation of focal adhesion kinase (FAK)/Rho-GTPase, PI3K/Akt/eNOS and matrix metalloproteinase-9 (MMP-9) molecular mechanism to promote the migration effect of WJCs. The higher level of integrins (??5β3 and CXCR4) was also induced by PU-Au after VEGF and SDF-1 stimulation. In addition, the EPCs with PU-Au were reduced thrombosis effectively and enhanced the differentiation of EPCs to endothelial cells. These results demonstrate that PU-Au might enhance cell proliferation and migration, and differentiate into endothelial cells contribute to neovascularization in the vascular lesion.
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