Antimelanogenesis effect of δ-tocotrienol on B16 melanoma cells

• Main Authors: Hsiu-wen Chen, 陳綉文
• Other Authors: Shu-jing Wu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/42401294641524802028

碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 99 === Several studies have shown that tocotrienols displayed the cholesterol lowering effect, antioxidative and anti-cancer activities. The study has not about antimelanogenesis mechanism (s) of δ-tocotrienol. So our study the effects of δT3 on skin-whitening agent and antimelanogenesis mechanism (s) in the first. In this study, our aims were (1) to evaluate the effect of α-, γ-, δ-tocotrienols (αT3, γT3, and δT3), and arbutin on cell viability, (2) to study the effects of δT3 and arbutin on the ROS production, and (3) to examine the effects of δT3 and arbutin on the melanin content, the expression of melanogenesis-related proteins such as tyrosinase, MC1R, Mitf, TRP (-1 and -2) and MAPK pathway in B16 melanoma cells. We found that δT3 possessed the best protective effect in B16 cells. Our data indicated that 20 μM δT3, but not arbutin, significantly decreased the generation of ROS, as a specific inhibitor of melanin production and reduced the expression of tyrosinase, MC1R, Mitf, TRP (-1 and -2) mRNA. Cells treated with δT3, and also markedly up-regulated the activation and phosphorylation of ERK kinases, down-regulated the phosphorylation of p38 kinase, and reduced melanin synthesis. These results suggest that δT3 performed well in both melanogenesis inhibition and antioxidation.