| Summary: | 碩士 === 中山醫學大學 === 醫學應用微生物研究所 === 99 === Influenza is a serious global public health problem that can cause economic burdens. Because of continually emerging drug-resistant influenza virus strains, developing new antiviral drugs is critical. Traditional Chinese medicines (TCMs) are broadly used in Asia as antipyretic and antiviral drugs for the treatment of colds, fever, and chronic bronchitis. This study developed an embryonated chicken egg model for evaluating 333 types of antiviral TCMs that act against influenza virus strains. Oseltamivir was used as a positive control against H1N1 in this model. Influenza A virus levels in allantoic fluid were titrated by conducting a hemagglutination test after incubation for 72 h at 35.5 °C and overnight at 4 °C. The cytotoxicity of the candidate TCM was assayed using the WST-1 system and expressed as CC50. Using this model, the EC50 of oseltamivir was 23.7 mg/kg. Twenty-two of 333 tested TCMs exhibited anti-influenza virus activity. The drugs included Rosaceae, Cornaceae, Dioscoreaceae, Rubiaceae, Compositae, Hypoxidaceae, Aristolochiaceae, Ranunculaceae, Equisetaceae, Araceae, Cruciferae, Scrophulariaceae, Gentianaceae, Labiatae, Violaceae, Convolvulaceae, and Liliaceae. The CC50 of the candidate drugs ranged from 0.22 mg/ml to 19.5 mg/ml. In conclusion, this model can be a valuable evaluation of in vivo pretesting of anti-influenza virus activity.
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