| Summary: | 碩士 === 中山醫學大學 === 免疫學研究所 === 99 === Microglia cell was a kind of glial cells with phagocytic function in the central nervous system (CNS). Typically, when brain was damaged, the activation of microglia cells released many kinds of cytokines which demonstrated phagocytosis, clearing cell debris, plaques and damaged neurons. However, the over-activation of microglia cells was harmful to the restoration of brain and caused the death of neuronal cells. Therefore, we hoped to observe whether it can inhibit the over-activation of microglia cells by researching the used of novel biomedical materials.
In this study, we mainly observed the effect of a novel nanomaterials alumina in phagocytosis. We cultivated microglia cells on the dish which was coated with alumina and added luciferin-expressing bacteria on the dish after stimulated by lipopolysaccharide (LPS). After that, we found that nanomaterials could inhibit phagocytosis. Moreover, we used the Western blotting to observe the possible path which LPS affected microglia cells on the dish coated with alumina. As a result, we found that the JNK expression of the group which used alumina was in a decreasing trend. In addition, we detected that alumina could reduce the activation of NF-κB by immunofluorescence staning assay. Finally, by using reverse transcription polymerase chain reaction (RT-PCR) method approach to observe the influences of alumina to TNF-α, IL-1β, the results indicated that the expression of them were all reduced. Therefore, we considered that the novel nanomaterials aluminum could inhibit the microglia cells'' abilities on phagocytosis and activation. Taken together, over-activation of microglia cell damaged nervous tissue; however, it might decrease the phagocytic function and over-activation through the usage of nanomaterial aluminum.
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