| Summary: | 碩士 === 輔英科技大學 === 醫事技術系碩士班 === 99 === Lung cancer is the leading cause of the cancer-related deaths. Tumor metastasis is very complex and this process requires a tumor cell to acquire enhanced motility, invasiveness and anoikis resistance to successfully establish a tumor at a distal site. Although cannabinoids are associated with antineoplastic activity in a number of cancer cell types, the effect in lung cancer cells has not been clarified. In the present study, we investigated the effects of a cannabinoid agonist, WIN 55212-2, on lung cancer cell proliferation and metastasis. The synthetic cannabinoid WIN 55212-2 inhibited the proliferation of human lung cancer cells, A549, in a dose-dependent manner. Cell wound healing assay showed WIN 55212-2 treatments inhibited the cell mobility of A549 cells. WIN 55212-2 had decreased levels of urokinase plasminogen activator (uPA), PAI-1 and ECM degradation-associated proteins including matrix metalloproteinase-2 (MMP-2) and TIMP-2. We also found that WIN 55212-2 treatment inhibited the NF-κB cis-reproting promoter activity. These findings suggest that the mechanism of WIN 55212-2 mediated anti-metastasis involves modulation of the expression of uPA、PAI-1、MMP-2, possibly by targeting NF-κB in A549 cells.
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