Detrimental Effect of Lead Exposure on Molar Development in Rat Fetuses
碩士 === 高雄醫學大學 === 牙醫學研究所 === 99 === Objective: This study evaluates effects of maternal exposure to different dose and time of lead on tooth development in rats fetuses. Materials &Methods: Pregnant rats were randomly divided into five groups: groups I was given deionized water, groups II and II...
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2011
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| Online Access: | http://ndltd.ncl.edu.tw/handle/44634748892200664477 |
| Summary: | 碩士 === 高雄醫學大學 === 牙醫學研究所 === 99 === Objective:
This study evaluates effects of maternal exposure to different dose and time of lead on tooth development in rats fetuses.
Materials &Methods:
Pregnant rats were randomly divided into five groups: groups I was given deionized water, groups II and III drank lead acetate water in dose of 50 and 200 mg/L respectively throughout gestation, groups IV and V drank lead acetate water in dose of 50 and 200 mg/L respectively throughout lactation. Rat fetuses were sacrificed at 35 days postnatally and first molar were collected for morphometric
studies.
Results:
No abnormalities in cusp number, form were noticed in the experimental groups. Many deep and longitudinal groves was noticed near enamo-dentinal junction on the molar surface. Analysis with ANOVA indicated a statically significant difference in linear measurement of crown size (P<0.0001). Further analysis revealed that the mesiodistal and buccolingual diameters were significantly smaller in lactation groups. Both bone morphogenetic protein-4 and fibroblast growth factor were immunohistochemical staining in stellate reticulum and ameloblast zone in the gestation groups and lactation groups. No differences were found in immunostaining intensity between the experimental groups and control groups.
Conclusions:
The administration of lead during gestation and lacation period cause adverse effect on molar size in the laboratory rat.
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