Multielemental analysis and arsenic speciation in betel nut and piper leaf by microwave assisted digestion, ion chromatography and inductively coupled plasma-mass spectrometry

• Main Authors: Chien-Che Sun, 孫健哲
• Other Authors: Yeou-Lih Huang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/07733590463213131375

碩士 === 高雄醫學大學 === 醫學檢驗生物技術學研究所 === 99 === Chewing betel quid will result in oral diseases and carcinoma. In Taiwan, where the practice of betel quid chewing is widespread, approximately two million people chew betel quid habitually. Recent studies have shown that betel quid chewing is an important risk factor for oral cancer similar to alcohol drinking and cigarette smoking. However, few studies have been devoted to the analysis of trace elements and metal species in betel nut and piper leaf. In the present study, a microwave-assisted digestion method with inductively coupled plasma mass spectrometry (ICP-MS) detection was developed for the simultaneous determination of arsenic (As), cadmium (Cd), cobalt (Co), molybdenum (Mo), silver (Ag), gallium (Ga) and lead (Pb) in the betel quid samples. In addition, we used HPLC coupled with ICP-MS for arsenic speciation in betel nut and piper leaf. Parameters such as acid/oxidizing reagents concentrations, acid/oxidizing solution volume, temperature, ramp time and hold time for the microwave-assisted digestion were investigated. Under the optimized conditions, the linearity, precision, accuracy and detection limit of the proposed methods were evaluated. The precision below 5.0 % RSD for the elements of interest was obtained. The accuracy evaluated with spike recovery and standard reference material (NIST SRM 1573a) was found to be acceptable. The limit of detection for As, Ag, Cd, Ga, Mo, Pb and Sn was 0.016, 0.040, 0.003, 0.020, 0.040, 0.010 and 0.170 µg/L, respectively. The analytical performance of the proposed method for arsenic speciation was evaluated and found to be reliable for the determination of arsenite, arsenate, monomethylarsonicacid, dimethylarsinic acid in betel nut and piper leaf. The levels of the seven elements of interest in this study were observed within the laws and regulations limiting value. Moreover, inorganic arsenic species was found to be the predominant arsenic species in piper leaf.