The Anti-diabetic Properties and Pharmacological Mechanisms of Green-processed Toona sinensis Extract
碩士 === 高雄醫學大學 === 醫學遺傳學研究所 === 99 === OBJECTIVE The leaves of Toona sinensis (TS) have been used as a nutritious food and an oriental medicine for a long time; however, the reported bioactive compounds of TS leaves are aqueous extract processed by using water or ethanol. The aims in this study are t...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2011
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| Online Access: | http://ndltd.ncl.edu.tw/handle/30049060379065150541 |
| Summary: | 碩士 === 高雄醫學大學 === 醫學遺傳學研究所 === 99 === OBJECTIVE The leaves of Toona sinensis (TS) have been used as a nutritious food and an oriental medicine for a long time; however, the reported bioactive compounds of TS leaves are aqueous extract processed by using water or ethanol. The aims in this study are to investigate the anti-diabetic properties and pharmacological mechanisms of the green-processed TS leaf extract (TS-G).
METHODS Type 2 diabetic mice model was used in this study. The biochemical data of mice was analysed by a Roche Cobas Integra 400 Chemistry Analyzer. Hematoxylin and eosin stains were used to observe the morphological changes of adipose and hepatic tissues. Real-time polymerase chain reaction was used to measure the related gene expression. Western blotting was used to measure the related protein expression.
RESULTS Both the green-processed leaf extract of TS (TS-G) and rosiglitazone treatments significantly prevented the elevation of blood glucose in animal study. The value of homeostasis model assessment of insulin resistance (HOMA-IR) was lower in the TS-G treated mice compared to the diabetic mice. The plasma levels of ALT and creatinine were not elevated by the TS-G treatment compared to the normal mice. The cell size of adipocytes were smaller in the TS-G treated mice compared to the diabetic mice. The ballooned hepatocytes showed in the diabetic mice but not in the TS-G and rosiglitazone treated mice. TS-G could not increase peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in adipose tissues.
CONCLUTIONS We provide evidence that TS-G prevented the progression of diabetes and the functional mechanisms of TS-G may not regulate through PPAR-γ pathway. Our findings suggest that the green-processed TS leaf extract may have potential in the development of anti-diabetic food supplements and medicines.
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