Enzymatic Carbon Dioxide Sequestration by using Carbonic Anhydrase

• Main Authors: Hsieh, Hsiao Hsin, 謝曉馨
• Other Authors: Chien, Liang jung
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/66162111712897707479

碩士 === 明志科技大學 === 生化工程研究所 === 99 === The increase in atmospheric concentrations of one of the vital green house gasses, carbon dioxide, due to anthropogenic interventions has led to several undesirable consequences such as global warming and related changes. In the global effort to combat the predicted disaster, several CO2 capture and storage technologies are being deliberated. One of the most promising biological carbon dioxide sequestration into bicarbonates which was endeavored. In this study, Carbonic anhydrase (icfA) from Synechocystis sp. PCC6803 was designed to be fused with (His)6 peptide at its C-terminus and silica-precipitating peptide R5 at its N-terminus. Once in contact with hydrolyzed tetramethoxysilane, the homogeneously dispersed purified dual-fusion R5-CA-(His)6 was biosilicification to form aggregates with size about 300nm and it can be reused at least 10 times and just a little activity loss. Analysis of enzyme kinetics data showed that the silica-encapsulated immobilized CA maintains high level of enzymatic activity. When operating the reactor at ambient temperature with silica-encapsulated immobilized carbonic anhydrase, the amount of CO2 initially containing~800 ppm CO2 was decreased to ~42 ppm in the scrubbed gas-a capture efficiency of ~94.7%；Without enzyme only 31% of initial CO2 was removed. The silica-encapsulated immobilized enzyme-based CO2 capture approach opens new opportunities to make capture CO2 processes more efficient and enable broader commercialization of technologies.