Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation
碩士 === 國立中興大學 === 化學工程學系所 === 99 === This study examined ultrasonic agitation extraction, column chromatography and supercritical anti-solvent(SAS) precipitation to perify five lignans from dried fruits of Schisandra chinensis.Column fractionation of the ultrasonic ethanol extraction resulted in a 1...
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ndltd-TW-099NCHU50630792017-10-29T04:34:09Z http://ndltd.ncl.edu.tw/handle/32681394308952544637 Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation 管柱流析劃分及超臨界抗溶純化北五味子果實木質酚素 Tai-Ling Huang 黃泰綾 碩士 國立中興大學 化學工程學系所 99 This study examined ultrasonic agitation extraction, column chromatography and supercritical anti-solvent(SAS) precipitation to perify five lignans from dried fruits of Schisandra chinensis.Column fractionation of the ultrasonic ethanol extraction resulted in a 10 times increasement of those five lignans and supercritical anti-solvent precipitation of the column elution resulted in 2.9 times increasing of the five lignans. Experiment data show that concentration of five lignans increasing from 26.14 mg/g in the extraction to 581.85 mg/g in the effluent after supercritical anti-solvent precipitation with a recovery of 84%, The elution contains schisandrol B of 145.32 mg/g, schisandrin A of 56.65mg/g, γ-schisandrin of 66.38mg/g , gomisin N of 266.70 mg/g and schisandrin C of 46.80 mg/g. Finally, experimental results of a response surface method designed for supercritical anti-solvent precipitations in the enhancement of the purity of five lignans showed that the SAS time and the carbon dioxide flow rate are significant in altering the purity and the recovery. This work demonstrated that the five lignans of Schisandra chinensis was successfully purified by using supercritical anti-solvent precipitation process. 張傑明 2011 學位論文 ; thesis 78 zh-TW |
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碩士 === 國立中興大學 === 化學工程學系所 === 99 === This study examined ultrasonic agitation extraction, column chromatography and supercritical anti-solvent(SAS) precipitation to perify five lignans from dried fruits of Schisandra chinensis.Column fractionation of the ultrasonic ethanol extraction resulted in a 10 times increasement of those five lignans and supercritical anti-solvent precipitation of the column elution resulted in 2.9 times increasing of the five lignans. Experiment data show that concentration of five lignans increasing from 26.14 mg/g in the extraction to 581.85 mg/g in the effluent after supercritical anti-solvent precipitation with a recovery of 84%, The elution contains schisandrol B of 145.32 mg/g, schisandrin A of 56.65mg/g, γ-schisandrin of 66.38mg/g , gomisin N of 266.70 mg/g and schisandrin C of 46.80 mg/g.
Finally, experimental results of a response surface method designed for supercritical anti-solvent precipitations in the enhancement of the purity of five lignans showed that the SAS time and the carbon dioxide flow rate are significant in altering the purity and the recovery. This work demonstrated that the five lignans of Schisandra chinensis was successfully purified by using supercritical anti-solvent precipitation process.
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author2 |
張傑明 |
author_facet |
張傑明 Tai-Ling Huang 黃泰綾 |
author |
Tai-Ling Huang 黃泰綾 |
spellingShingle |
Tai-Ling Huang 黃泰綾 Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
author_sort |
Tai-Ling Huang |
title |
Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
title_short |
Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
title_full |
Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
title_fullStr |
Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
title_full_unstemmed |
Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation |
title_sort |
purification of lignans from schisandra chinensis frutus by using column elution frationantion and supercritical antisolvent precipitation |
publishDate |
2011 |
url |
http://ndltd.ncl.edu.tw/handle/32681394308952544637 |
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