Purification of Lignans from Schisandra Chinensis Frutus by Using Column Elution Frationantion and Supercritical Antisolvent precipitation

• Main Authors: Tai-Ling Huang, 黃泰綾
• Other Authors: 張傑明
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/32681394308952544637

碩士 === 國立中興大學 === 化學工程學系所 === 99 === This study examined ultrasonic agitation extraction, column chromatography and supercritical anti-solvent(SAS) precipitation to perify five lignans from dried fruits of Schisandra chinensis.Column fractionation of the ultrasonic ethanol extraction resulted in a 10 times increasement of those five lignans and supercritical anti-solvent precipitation of the column elution resulted in 2.9 times increasing of the five lignans. Experiment data show that concentration of five lignans increasing from 26.14 mg/g in the extraction to 581.85 mg/g in the effluent after supercritical anti-solvent precipitation with a recovery of 84%, The elution contains schisandrol B of 145.32 mg/g, schisandrin A of 56.65mg/g, γ-schisandrin of 66.38mg/g , gomisin N of 266.70 mg/g and schisandrin C of 46.80 mg/g. Finally, experimental results of a response surface method designed for supercritical anti-solvent precipitations in the enhancement of the purity of five lignans showed that the SAS time and the carbon dioxide flow rate are significant in altering the purity and the recovery. This work demonstrated that the five lignans of Schisandra chinensis was successfully purified by using supercritical anti-solvent precipitation process.