A study of the practicability of using disposable electrochemical biosensor to detect α-methylacyl-CoA racemase

• Main Authors: Kai-Lun Cheng, 鄭凱倫
• Other Authors: 薛富盛
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/jctm2y

碩士 === 國立中興大學 === 材料科學與工程學系所 === 99 === α-methylacyl-CoA racemase (AMACR) was recently found the newest biomarker for prostate cancer. The purpose of this study is to develop an AMACR biosensor on the basis of the iridium nano-particle based single-use, dioposable biosensor made in our lab. Using pristanic acids, a chemical naturally has stereoisomers, mixed with CoA (Coenzyme A), Mg2+, ATP (Adenosine 5’-triphosphate,ATP) to generate the substrate, pristanoyl-CoA. AMACR could transfer (2R)-isomer to (2S)-isomer, which is further react with peroxisomal acyl-coenzyme A oxidase 3 (Acox3) and produce the end product H2O2. In a fixed concentration of pristanic acids and fixed reaction time with enzymes could quantitative the concentration of AMACR. The H2O2 is monitored by amperometric tests at an operating potential of +0.45V versus Ag/AgCl reference electrode. The result comes out that the best incubation time for the transformation from pristanic acid to pristanoyl-CoA is three days. Electrochemical measurement shows this disposable biosensor is capable of detecting the oxidizing current of H2O2, and has good linear relationship between response current and AMACR concentration in phosphate buffer solution. The best pH value for operating this sensor is pH7.0.