cDNA cloning and expression of the vitellogenin receptor from the oriental fruit fly, Bactrocera dorsalis

• Main Authors: Wei-Jen Lin, 林威仁
• Other Authors: Mei-Er Chen
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/67939185956929578443

碩士 === 國立中興大學 === 昆蟲學系所 === 99 === The vitellogenin (Vg) is absorbed into the growing oocytes via receptor-mediated endocytosis for the embryonic development. In this study, vitellogenin receptor cDNA of the oriental fruit fly (Bactrocera dorsalis Hendel) was cloned and its expression was analyzed. Using RACE technology, a putative B. dorsalis vitellogenin receptor (BdVgR) was cloned. The BdVgR cDNA has a length of 6585 bp encoding 1923 amino acids. The BdVgR has conserved motif arrangement with other insect VgRs, and showed the high similarity to Drosophila VgR (70%). The PCR analysis showed that the mRNA of BdVgR was expressed in both ovary and fat body. The western blot analysis demonstrated that the BdVgR (~213 kDa) was expressed in both ovary and fat body. The qRT-PCR analysis showed that the expression of BdVgR in the fat body was low after eclosion.The BdVgR mRNA increased from day 4, and it showed the highest level on day 6, and then it decreased with time. The expression level of BdVgR in the ovary was high in 24h after eclosion, and then it decreased until day 2. It increased with time on day 3. The BdVgR protein was expressing highly in the fat body between day3 and day 6; but it gradually increased from day 3 in the ovary. In conclusions, the BdVgR was expressed during the ovary development. The function of BdVgR in the fat body could be involved in reuptake vitellogenins from the hemolymph.