Immunomodulatory effect of Bifidobacterium logum subsp. infantis GB-1496 in mite allergen-sensitized murine model

• Main Authors: Szu-Chi Huang, 黃思齊
• Other Authors: Meei-Yn Lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/34461619369098705314

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 99 === Over the years, lactic acid bacteria have long been regarded as the effectiveness of probiotics, which can maintain of intestinal microflora, reduce lactose intolerance, blood pressure, blood fats, anti-cancer, anti-inflammation and regulate the immune system. It is kind of functional food is widely liked by people. Bifidobacterium, is belong to lacitic acid bacteria family and the greatest microflora in the infant gut. In the studies found that the population of Bifidobacterium in the gut is associated with the life span of the host, and the more longevity, the higher proportion of this bacteria.The main function of Bifidobacterium is to treat diarrhea, maintain intestinal microbial homeostasis and protect against harmful intestinal bacteria colonizing. Although there have confirmed their effectiveness in the immune experiments , but generally found that their ability to regulate immunity is not particularly powerful.The previous experiments has been shown that Bifidobacterium longum supsp. infants. GB-1496 had best capacity to modulate immunity in vitro. Therefore, further investigations on regulation of immune responses in murine model in vivo. In the present studies, we fed the Der p-sensitized BALB/c mice on high (3.0*107 cfu/per mice) , medium (9*107 cfu/per mice) and low (3.0*106 cfu/per mice) dose. The proliferation of splenocyte, cytokines secreted by splenocyte (IFN-γ, IL-4, IL-10 and IL-17) , and specific immunoglobulin in serum were measured. The results showed that only medium dose group stimulated by ConA had significant enhance proliferation of splenocyte; high and mediumn dose group can significantly increase the production of IFN-γ, reduced the secretion of IL-4 and no effect of IL-10 and IL-17; IFN-γ/IL-4 ratio is also significantly improved, so that the immune response directed Th1; specific immunoglobulin trial, three doses were significantly lower specific IgE, and High dose can down regular specific IgG1. These results showed that Bifidobacterium longum subsp. infants GB-1640. had ability to modulate in mite allergen-sensitize mice.