SNP Genotypes of GH, KRT5, RPS8 and COL1A1 Genes Associated with Velvet Antlers Traits in Formosan Sambar

• Main Authors: Wei-Jyun Chen, 陳韋君
• Other Authors: 黃木秋
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/88956796323245788280

碩士 === 中興大學 === 動物科學系所 === 99 === Formosan sambar (Rusa unicolor swinhoei) is endemic subspecies in Taiwan. The price of the velvet antlers is higher than other livestock products. The study was performed to analysis the genotypes in association with velvet antlers production of the GH (growth hormone), KRT5 (keratin 5), RPS8 (ribosomal protein S8), and COL1A1 (collagen, type I, alpha 1) genes by LIS-SSCP and PCR-RFLP. The specific primers were designed by Primer Express software to amplify the target DNA fragments from Formosan sambar genomic DNA by PCR, and followed by SSCP analysis. Single nucleotide polymorphisms (SNPs) were found by sequencing technology, and then processed PCR-RFLP and multiplex minisequencing method for genotyping. Further study was to investigate the correlation between genotypes and performance in Formosan sambar by SAS program. The results showed that, in the SNP C222T of GH gene, the velvet antler weight in 1st-10th saw with CC type seemed to higher than the CT and TT types in Formosan sambar (P < 0.20) , and velvet antler weight in 1st-3rd saw with CC type was significant higher than the CT and TT types (P < 0.05) ; In the SNP G323A, the velvet antler weight in 1st-3rd saw with AA type seemed to higher than the GA and GG types, but in 4th-6th saw with GA type seemed to higher than the GG type, and in 7th-10th saw with GA type seemed to higher than the AA type (P < 0.20). In the SNP C88T of RPS8 gene, the velvet antler weight in 1st-3rd saw with CC type seemed to higher than the TT type (P < 0.20). The velvet antler weight in 1st-10th saw of CC type of COL1A1 gene seemed to higher than the TT type, but in 7th-10th saw, CC type seemed to higher than the CT type (P < 0.20). These genes may involve in the formation of velvet antler, but need increase the sample size to confirm the results. The results may be useful in Formosan sambar breeding.