Hybridization of Kalanchoe Species Between Different Sections

• Main Authors: Chia-Wei Wang, 王嘉偉
• Other Authors: 朱建鏞
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/52401116734729721140

碩士 === 國立中興大學 === 園藝學系所 === 99 === In this research, Kalanchoe cutivars： Kalanchoe manginii ‘Wendy’, K. blossfeldiana ‘Isabella’, species：K. pinnatum, K. nyikae, the inbred Lines of K. garambiensis ‘green leaf’ and K. garambiensis ‘purple leaf’: KG 93-13, KGP 93-31 and the inter-sectional hybrids between K. manginii ‘Wendy’, K. pinnatum which belong to sect. Bryophyllum and K. garambiensis ‘green leaf’, K. garambiensis ‘purple leaf’, K. blossfeldiana Poellnitz ‘Isabella’ which belong to sect. Kalanchoe: ‘KGP × KP 93-1’, ‘Isa × KP 94-1’, ‘Isa × KP 94-3’, ‘Isa × Wendy 94-1’, ‘Isa × Wendy 94-2’, ‘(KG93-13 × KP) 98-1’, ‘(KG93-13 × KP) 98-2’ were subjected to GA3 treatment, pollen viability test, inter-sectional hybridization, backcross, and examine the plant and flower characteristics. Days to flower of the inter-sectional hybrids ranged from 117.6~128.3 days, and spaying of GA3 could shorten the days to flower about 5.7~10 days. Pollens that cultured on B&K medium containing 5% sucrose and kept at 25℃ for two hours, got the highest germination rate in K. garambiensis ‘green leaf 93-13’, about 63.3%, followed by K. pinnatum 59.9%, and the inter-sectional hybrids‘Isa × Wendy 94-1’,‘Isa × Wendy 94-2’ did not germinate. The inter-sectional cross between K. pinnatum and K. garambiensis ‘green leaf 93-13’, when K. pinnatum was used as maternal parent, the fruit set was 75% and fertility rate was 11.8%, but the seeds did not germinate. In the reverse crosses, when K. garambiensis ‘green leaf 93-13’ was used as maternal parent, the fruit set was 70%, fertility rate was 10.4% and germination rate was 0.9%. Only the backcross of‘KGP × KP 93-1’, ‘KGP × KP 93-1M1’ and ‘(KG93-13 × KP) 98-1’could obtain seeds, however, the seeds did not germinate. And the embryo culture also could not get the germinative seeds. The Morphological characters of the inter-sectional hybrids are mostly intermediate between parents, and showed hybrid vigor in the number of flower, which was more than either parent.The epiphyllous bud formation on the leaf margin was one of the conspicuous characteristics of K. pinnatum, but all the hybrids of K. pinnatum did not present this characteristics.