| Summary: | 碩士 === 國立中興大學 === 獸醫學系暨研究所 === 99 === There are three objects of this study: to establish hematologic and biochemical reference values in red-eared sliders, hematologic and biochemical data analysis in hooked red-ear sliders, factors associated with successful retrieval fishhook by endoscopy or surgery. In hematologic and biochemical reference values establishment, eighteen normal red-eard sliders from artificial lakes in central region were used in this research. Blood samples were obtained from caudal venous sinus. 95% confidence interval was used for reference interval. Blood samples from sixteen hooked red-eared sliders from artificial lakes in central region were used for hematologic and biochemical analysis. Blood was taken before surgery, one day, and seven days after surgery. When the hematologic and biochemical data from hooked red-eared sliders before surgery to normal ones were compared, it showed significant elevations of lymphocyte ratio and γGT; reductions of white blood cell count, heterophil ratio, red blood cell count, hemoglobin, packed cell volume and globulin. It suggested the existence of fishhook in red-eared sliders would cause reduction of immunity, chronic blood loss and fatty liver. Prognosis was not related to preoperative blood values. After endoscopic surgery, elevations of heterophils, AST, ALT, LDH, CK, γGT, BUN, creatinine and uric acid were observed in hooked red-eared sliders. These results indicated that endoscopic surgery can cause inflammation, stress and tissue damage. In the part of factors associated with successful retrieval fishhook by endoscopy or surgery, twenty-four hooked turtles were examined during the study period. Twenty-one of these were obtained from artificial lakes in central region. Three of these were found with a fishing line extended from the oral cavity and radiographs diagnosed fishhook ingested. Techniques used to remove fishhooks were classified as endoscopic removal and surgical removal. Duration of endoscopic retrieval of fishhook from either esophagus, cardia and stomach has no significant difference when compared to duration of surgical retrieval of fishhook from esophagus. In esophagus, cardia and stomach, whether a fishhook could be endoscopically successfully retrieved was not related to its length, width, orientation, barb, or length of fishing line. Surgical successful rate was also not related to the depth of fishhook. Endoscopic retrieval time and fishhook- related factors were not significantly related. Once the fishhooks are ingested by wild chelonian, we still suggest that the fishhook should be removed. Although the endoscopic successful rate and the duration of retrieval were not related to all fishhook-related factors, preoperative radiology examinations still provide the orientation and location of fishhooks, which helps to retrieve fishhooks by endoscopy. Endoscopic retrieval of foreign body is the first choice if the equipments are available. Fishhooks should not be forced removed by endoscopy when they are embedded deeply in the tissue, otherwise it may lead to death. Surgical removal is then recommended if endoscopic retrieval is unsuccessful. However, when fishhook located near cardia and surrounded by a lot of hard connective tissue, surgery is then not suitable for this kind of chelonian. The coexistence of chelonian and the fishhook would be suggested.
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