Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides
碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 99 === High diversity and multiple serotypes in bacteria are the main bottleneck of cross-protective vaccine development. In previous studies, our lab isolated three protective immunogens, HSP60, enolase and GAPDH in Photobacterium damselae subsp. Piscicida (Ph....
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ndltd-TW-099NCKU51110032015-10-30T04:05:21Z http://ndltd.ncl.edu.tw/handle/81144253900163449187 Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides 比較巴斯德桿菌與坎氏弧菌之共同免疫原對於石斑魚抵抗坎氏弧菌感染之交叉保護能力研究 Chin-JungChang 張晉榮 碩士 國立成功大學 生物科技研究所碩博士班 99 High diversity and multiple serotypes in bacteria are the main bottleneck of cross-protective vaccine development. In previous studies, our lab isolated three protective immunogens, HSP60, enolase and GAPDH in Photobacterium damselae subsp. Piscicida (Ph. d. p.) by immunoproteomics approach. In other studies, Vibrio campbellii was identified as a pathogen in fish farm in Tainan. These three immunogens were also isolated from this V. campbellii by the same approach. Preliminary data showed that the same immunogens derived from Ph. d. p. and V. campbellii shares at least 84 % nucleotide identity and 94 % amino acid homology. HSP60, enolase and GAPDH were considered as high conserved immunogens. We evaluated the feasibility of using these three immunogens as universal vaccine candidates in this research. Orange-spotted grouper were immunized with E.coli expressing rHSP60, rEnolase and rGAPDH derived from Ph. d. p. and V. campbellii, and challenged with V. campbellii. Results indicated that rHSP60, rEnolase and rGAPDH of Ph. d. p. did not protect V. campbellii challenge, indicating antigens even contain 94 % amino acid similarity, still could not provide cross-protectivity. Homology modeling prediction results indicated that the difference of amino acids sequences of these three pairs of proteins located on their surface. We suggested the variable surface structure may be the key in determining the response of immunity. Huey-Lang Yang 楊惠郎 2011 學位論文 ; thesis 115 zh-TW |
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碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 99 === High diversity and multiple serotypes in bacteria are the main bottleneck of cross-protective vaccine development. In previous studies, our lab isolated three protective immunogens, HSP60, enolase and GAPDH in Photobacterium damselae subsp. Piscicida (Ph. d. p.) by immunoproteomics
approach. In other studies, Vibrio campbellii was identified as a pathogen in fish farm in Tainan. These three immunogens were also isolated from this V. campbellii by the same approach. Preliminary data showed that the same immunogens derived from Ph. d. p. and V. campbellii shares at least 84 % nucleotide identity and 94 % amino acid homology. HSP60, enolase and GAPDH were considered as high conserved immunogens.
We evaluated the feasibility of using these three immunogens as universal vaccine candidates in this research. Orange-spotted grouper were immunized with E.coli expressing rHSP60, rEnolase and rGAPDH derived from Ph. d. p. and V. campbellii, and challenged with V. campbellii. Results indicated that rHSP60, rEnolase and rGAPDH of Ph. d. p. did not protect V. campbellii challenge, indicating antigens even contain 94 % amino acid similarity, still could not provide cross-protectivity.
Homology modeling prediction results indicated that the difference of amino acids sequences of these three pairs of proteins located on their surface. We suggested the variable surface structure may be the key in determining the response of immunity.
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author2 |
Huey-Lang Yang |
author_facet |
Huey-Lang Yang Chin-JungChang 張晉榮 |
author |
Chin-JungChang 張晉榮 |
spellingShingle |
Chin-JungChang 張晉榮 Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
author_sort |
Chin-JungChang |
title |
Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
title_short |
Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
title_full |
Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
title_fullStr |
Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
title_full_unstemmed |
Compare the cross protectivity of shared immunogens from Photobacterium damseale subsp. piscisida and Vibrio campbellii on Vibrio campbellii infection in Epinephelus coioides |
title_sort |
compare the cross protectivity of shared immunogens from photobacterium damseale subsp. piscisida and vibrio campbellii on vibrio campbellii infection in epinephelus coioides |
publishDate |
2011 |
url |
http://ndltd.ncl.edu.tw/handle/81144253900163449187 |
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