| Summary: | 碩士 === 國立體育大學 === 運動科學研究所 === 99 === Yi-Yi-Ren-Tang is a Traditional Chinese Medicine preparation for treatment and attenuation of arthritis and muscle aches. Ephedrae Herba is one of the prescriptions of Yi-Yi-Ren-Tang. The major compositions of Ephedrae Herba, ephedrines, are banned substances on the World Anti-Doping Agency (WADA) list. The purpose of this study was to investigate the elimination of urinary ephedrines after administering Yi-Yi-Ren-Tang. Eight healthy volunteers, with a mean age (± SD) of 25.88 (± 2.85) years, weight of 65.00 (± 11.35) kg, height of 171.88 (± 7.34) cm, participated in the experiment. The volunteers took a single dose of 2 g Yi-Yi-Ren-Tang, all urine was collected at certain intervals after 48 hours of administration. Ten healthy athletes were divided into continuously three days or six days groups which took a dose of 2 g for Yi-Yi-Ren-Tang three times per day for three or six days. The subjects in group of continuously three days were 19.33 (± 1.37) years old, 66.83 (± 10.83) kg, and of 159.67 (± 6.47) cm. The subjects in group of continuously six days were 20.50 (± 1.73) years old, 73.57 (± 9.00) kg, and 159.00 (± 6.06) cm. All urine was collected for six days and nine days after administration. Urinary ephedrines for all urine samples were analyzed by liquid chromatography tandem mass spectrometry. The results showed that the maximum concentrations of ephedrine (E), methylephedrine (ME), norephedrine (NE), norpseudoephedrine (NPE), and pseudoephedrine (PE) following single dose administration were 87.83 ng/mL, 111.11 ng/mL, 155.26 ng/mL, 82.44 ng/mL and 69.41 ng/mL, respectively. The Tmax of ephedrines were between 1-1.4 hours, the t1/2 was between 6-7 hours. The maximum concentrations of ephedrine, methylephedrine, norephedrine, norpseudoephedrine, and pseudoephedrine following three days continuously were 336.00 ng/mL, 266.00 ng/mL, 286.00 ng/mL, 293.61 ng/mL and 314.00 ng/mL, respectively. The maximum concentrations following six days continuously were 318.00 ng/mL, 311.00 ng/mL, 301.67 ng/mL, 155.00 ng/mL and 246.00 ng/mL, respectively. All the concentration of the urinary ephedrines were lower than the threshold of WADA. This study concluded if the athlete takes the Yi-Yi-Ren-Tang preparation according to the drugs instructions (2 g, three times a day) for short-term (six days) in competition would not lead to positive doping result.
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