Defining the Functional Components of Tetracycline-derived Dengue Virus Type 2 Inhibitor

• Main Authors: Liu, Ching-Hung, 劉慶鴻
• Other Authors: Yang, Yun-Liang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/66393324765207238360

碩士 === 國立交通大學 === 生物科技學系 === 99 === Dengue fever has become a major global health issues in tropical and subtropical regions. And it is considered the most important arthropod-born viral disease affecting humans in terms of morbidity and mortality. About 50-100 million cases of illnesses occur each year, and more than 100 countries have endemic dengue transmission. In previous works, researchers focused on dengue prevention and vaccine, and some targeted the enzymes for dengue replication. But there are still no commercial available drugs or vaccines for dengue disease. Previously, our laboratory has discovered that tetracycline derivatives can be need as inhibitors on dengue viruses. But not all of tetracycline derivatives are effective on the virus. Based on previous resuls, I selected a series of tetracycline derivatives and analogues to determine their effect on virus infectivity. My result shows all tested tetraccyclines have inhibitory effects on DV to various extent. Their effect on dengue virus serotypeⅡ is specific. In addition, I use immunofluorescence labeling detect to the presence of viral protein in the hosts and the presence of host nuclei. The results confirmed that the drugs have inhibitory effect on DV and low toxicity to the host cell.