| Summary: | 碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 99 === Vibrio parahaemolyticus is one of pathogens in the human intestines. Consumption of seafood contaminated with Vibrio parahaemolyticus usually leads to the development of acute gastroenteritis. Food poisoning via Vibrio parahaemolyticus has been reported throughout the world and particularly severe in Asia. Ususlly diagnosis of Vibrio parahaemolyticus is performed through biological method like plating which takes days. Biosensors provide alternative detection methods within 30 minutes. This study utilized the microelectrode-dielectrophoresis impedance biosensor to detect aggregated Vibrio parahaemolyticus on the surface of the chip by dielectrophoresis. The area of the maximum electrode of the sensor is 0.19 mm2, and this dielectrophoresis impedance biosensor did not require the addition of any redoxs probe in the system. This biosensor then utilized dielectrophoresis to manipulate and concentrate the Vibrio parahaemolyticus in the solution. First of all, the results of dielectrophoresis were theoretically simulated. Subsequently, polystyrene beads and Vibrio parahaemolyticus were manipulated by dielectrophoresis. And, Vibrio parahaemolyticus were detected by the impedance sensor subsequently. This study also investigated the effect of electrode size. To improve the sensitivity and specificity of detection, the surface of chip was modified with anti-Vibrio parahaemolyticus antibodies. The data from impedance measurements were analyzed and an equivalent circuit was built. Based on the model, solution resistance dominates the impedance change caused by the detection of Vibrio parahaemolyticus. The microelectrode-dielectrophoresis impedance biosensor detected a minimum of 1×10^5CFU/ml of Vibrio parahaemolyticus without use of micro-fluidic channel.
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