Effects of light intensity and light wavelength on the production of active polysaccharides by Chlorella sp. in batch cultures

• Main Authors: Kun-yan Chen, 陳昆延
• Other Authors: Chin-hang Shu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/85083758274940982358

碩士 === 國立中央大學 === 化學工程與材料工程研究所 === 99 === Chlorella sp. has used in the commercialization production because of it has many nutrition and easy to culture. In recent years, because the energy crisis reappears and Chlorella sp. can produce biodiesel, Chlorella sp. becomes the research key point of various countries. In addition, people increased to the demand of anticancer treatment medicine, so the research about Chlorella sp. takes seriously because it can produce activated polysaccharides. Chlorella sp. has the chlorophyll and is possible to absorb the light energy so light is a important factor effecting Chlorella sp. growth. This research had studied illumination influence in Chlorella sp. growth. Experimental used different light intensity, included 1000, 2000, 4000, 6000, 8000, 10000 Lux to culture Chlorella sp. and produce polysaccharides, then used colored light, blue LED and red LED, culture Chlorella sp.. In light intensity experiment, polysaccharides production increased with light intensity raises, Chlorella sp. has the maximum specific growth rate 0.007 hr-1 in 4000 Lux. In polysaccharides production, 6000 Lux has the maximum extracellular polysaccharides 30.41 mg L-1 and 8000 Lux has the maximum intercellular polysaccharides 52.12 mg L-1. In colored light experiment, red light can promote Chlorella sp. growth and blue light promote product production, Both''s best light intensity is 1000 Lux. In biological activity experiment, intercellular polysaccharides’s biological activity was better than extracellular polysaccharides. The best biological actived polysaccharides was intercellular polysaccharides culturing under 8000 Lux, which promoted the intercellular polysaccharides activity 22 % than Spirulina intercellular polysaccharides.