Investigation of the properties of single erythrocyte using PDMS microfluidic channels

• Main Authors: Chen Da Lin, 林辰達
• Other Authors: Jong Ching Wu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/53267606586383353611

碩士 === 國立彰化師範大學 === 物理學系 === 99 === The poly(dimethylsiloxane) (PDMS) microfluidc channel fabricated by soft lithography were applied to study the deformation and erythrocytes sedimentation rate (ESR) of single human red cell. In the soft lithography technique, the master was made from SU-8 photoresist to obtain vertical sidewall using light source with wavelength at 365 nm. After casting and curing process, PDMS replica is peeled from the master and treats with O2 plasma to bond with glass. The width (w) and height (h) of this microfluidic channel are controlled to be 10 m and 2.5 m, respectively, to make red blood cells flow in the channel without turning over and can be visualized more clearly. This configuration is useful to investigate the erythrocytes sedimentation rate (ESR) and the deformation under a static pressurization of single cell.